anti col iii monoclonal antibody  (Servicebio Inc)

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA-10 Family Promotes Renal Fibrosis through the VASH-1/Smad3 Pathway

doi: 10.3390/ijms25105232

Figure Lengend Snippet: Depletion of miR-10a and miR-10b alleviates RF and Smad3 phosphorylation induced by UUO. ( A ) The process of obtaining KO mice through hybridization. ( B ) Genotype identification: comparison of miR-10a and miR-10b gene-deficient mice with WT mice. ( C ) Representative images (superincumbent image scale bars, 50 µm) of kidney sections from the UUO model stained with H&E and Masson, showing day 7 and day 14; sham serves as the control. ( D ) Expression results of Fibronectin, Col-III, and p-Smad3 in Western blot experiments, with β-actin as the control. ( E ) Expression results of Fibronectin and Col-III in RT-qPCR experiments, with β-actin as the control. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Louis, MO, USA) via electrophoresis, followed by blocking with 5% BSA at room temperature for 2 h. Primary antibodies for each protein were diluted in 5% BSA as follows: Fibronectin (1:1000, 26836S, CST), Col-III (1:1000, 66887S, CST), VASH-1 (1:1000, sc-365541, Santa Cruz), p-Smad3 (1:1000, 9520S, CST), Smad3 (1:1000, 9513S, CST), β-actin (1:1000, #AF7018, Affinity), and EGFP (1:5000, SRP15324, Saier); the secondary antibodies used were anti-rabbit (1:40,000, S0001, Affinity) and anti-mouse (1:40,000, S0002, Affinity).

Techniques: Phospho-proteomics, Hybridization, Comparison, Staining, Control, Expressing, Western Blot, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA-10 Family Promotes Renal Fibrosis through the VASH-1/Smad3 Pathway

doi: 10.3390/ijms25105232

Figure Lengend Snippet: Overexpression of miR-10 promotes TGF-β1-induced fibrosis and Smad3 phosphorylation in HK-2 cells. ( A ) Morphological changes in HK-2 cells under 20 ng/mL TGF-β1 stimulation. ( B ) Western blot analysis of Fibronectin, Col-III, and p-Smad3 expression in HK-2 cells under TGF-β1 stimulation, with β-actin as a control. ( C ) RT-qPCR analysis of miR-10a, miR-10b, Fibronectin, and Col-III expression in HK-2 cells under TGF-β1 stimulation, with β-actin as a control. ( D ) Western blot examination of relevant target molecules in HK-2 cells transfected with the corresponding plasmids under TGF-β1 stimulation. * p < 0.05, ** p < 0.01, and **** p < 0.0001.

Article Snippet: Louis, MO, USA) via electrophoresis, followed by blocking with 5% BSA at room temperature for 2 h. Primary antibodies for each protein were diluted in 5% BSA as follows: Fibronectin (1:1000, 26836S, CST), Col-III (1:1000, 66887S, CST), VASH-1 (1:1000, sc-365541, Santa Cruz), p-Smad3 (1:1000, 9520S, CST), Smad3 (1:1000, 9513S, CST), β-actin (1:1000, #AF7018, Affinity), and EGFP (1:5000, SRP15324, Saier); the secondary antibodies used were anti-rabbit (1:40,000, S0001, Affinity) and anti-mouse (1:40,000, S0002, Affinity).

Techniques: Over Expression, Phospho-proteomics, Western Blot, Expressing, Control, Quantitative RT-PCR, Transfection

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA-10 Family Promotes Renal Fibrosis through the VASH-1/Smad3 Pathway

doi: 10.3390/ijms25105232

Figure Lengend Snippet: MiR-10 family promotes fibrosis by modulating the VASH-1/Smad3 pathway. ( A ) Western blot results of VASH-1 in mouse kidneys, with β-actin as a control. ( B ) After stimulation with 20 ng/mL TGF-β1, Western blot and RT-qPCR were performed to detect VASH-1 expression in HK-2 cells, with β-actin as a control. ( C ) After the transfection of HK-2 cells with overexpression plasmids, the results of Western blot and RT-qPCR experiments on the expression of VASH-1, with β-actin as a control, were determined. ( D ) After the transfection of HK-2 cells with knockdown plasmids, the results of Western blot and RT-qPCR experiments on the expression of VASH-1, with β-actin as a control, were determined. ( E ) Under stimulation with 20 ng/mL TGF-β1, after transfection with VASH-1-related plasmids, the protein expression results of Fibronectin, Col-III, and p-Smad3 in HK-2 cells, with β-actin as a control, were determined. ( F ) Under stimulation with 20 ng/mL TGF-β1, after transfection with relevant plasmids, the protein expression results of Fibronectin, Col-III, and p-Smad3 in HK-2 cells, with β-actin as a control, were determined. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Louis, MO, USA) via electrophoresis, followed by blocking with 5% BSA at room temperature for 2 h. Primary antibodies for each protein were diluted in 5% BSA as follows: Fibronectin (1:1000, 26836S, CST), Col-III (1:1000, 66887S, CST), VASH-1 (1:1000, sc-365541, Santa Cruz), p-Smad3 (1:1000, 9520S, CST), Smad3 (1:1000, 9513S, CST), β-actin (1:1000, #AF7018, Affinity), and EGFP (1:5000, SRP15324, Saier); the secondary antibodies used were anti-rabbit (1:40,000, S0001, Affinity) and anti-mouse (1:40,000, S0002, Affinity).

Techniques: Western Blot, Control, Quantitative RT-PCR, Expressing, Transfection, Over Expression, Knockdown